Recovery of Proteins and Enzyme Activity (DEAE-825)

The tables show good recovery of IEC DEAE-825 (a column for ion exchange chromatography) for proteins and enzyme activity.

Recovery of proteins
Protein Recovery (%)
Trypsin inhibitor 96
beta-Lactoglobulin 100
Ovalbumin 101
Myoglobin 100
gamma-Globulin 99
BSA 97
Thyroglobulin 90
Recovery of enzyme activity
Enzyme Recovery (%)
Kallikrein 92
Lipoxidase 96
Catalase 94

Sample load : 0.5mg each

Column : Shodex IEC DEAE-825 (8.0mmID*75mm)
Eluent : (A); 20mM Tris-HCl buffer(pH8.0)
         (B); (A) + 0.5M NaCl
         Linear gradient: 0min to 1min, (A) to (B)