Recovery of Proteins (KW-803)
Both PROTEIN KW-803 and Company A column (a column for aqueous SEC (GFC)) show high mass recoveries of ribonuclrease A and thyroglobulin. Such high recoveries are attributed to minimum adsorption properties. Furthermore, the calibration curve obtained by sample loading and peak areas shows good linearity in a wide range between 1 micro-g to 100 micro-g.
| Sample | Column | 1 micro-g | 5 micro-g | 10 micro-g | 50 micro-g | 100 micro-g |
|---|---|---|---|---|---|---|
| Ribonuclease A | KW-803 | 108.6 | 96.3 | 98.2 | 98.8 | 99.0 |
| Company A | 110.5 | 100.6 | 97.7 | 98.3 | 98.9 | |
| Thyroglobulin | KW-803 | 106.1 | 98.2 | 97.3 | 98.7 | 98.6 |
| Company A | 111.9 | 92.6 | 93.9 | 95.9 | 96.6 |
 |
 |
Columns : Shodex PROTEIN KW-803 (8.0mmID*300mm), Company A column Eluent : 50mM Sodium phosphate buffer(pH7.0) + 0.3M NaCl Flow rate : 1.0mL/min Detector : UV(220nm) Column temp. : Room temp.