Reversed Phase, Hydrophilic Interaction and Normal Phase Chromatography Columns
To check the deterioration of column performance, measure the column plate number and compare it with the number written in the inspection data sheet which is attached to the column. The test conditions for calculation of the plate number is determined for each column type and shown in the table below.
When you first use a column, we recommend you to measure its plate number before use. Plate number (N) can be caluculated by the formula in the right figure.
For the columns whose test conditions are shown with blue letters, the column performance should be measured by resolution, and not plate number.
| Column type |
Test conditions
(1)Sample (2)Injection volume (3)Eluent (4)Flow rate (5)Temperature (6)Detector |
|---|---|
| ODP-50 6D ODP-50 6E ODP-50 4B ODP-50 4D ODP-50 4E ODP-50 2D |
(1)0.75micro-L/mL n-Hexyl benzoate(ODP-50 6D, ODP-50 6E, ODP-50 4B, ODP-50 4D, ODP-50 4E) 0.08% n-Hexyl Benzoate(ODP-50 2D) (2)10micro-L(ODP-50 6D), 17micro-L(ODP-50 6E), 3micro-L(ODP-50 4B), 6micro-L(ODP-50 4D, ODP-50 4E.), 7micro-L(ODP-50 2D), (3)CH3CN/H2O=65/35 (4)1.0mL/min(ODP-50 6D, ODP-50 6E), 0.6mL/min(ODP-50 4B, ODP-50 4D, ODP-50 4E), 0.2mL/min(ODP-50 2D) (5)30deg-C(ODP-50 6D, ODP-50 6E, ODP-50 4B, ODP-50 4D, ODP-50 4E), Ambient(ODP-50 2D) (6)UV(254nm) |
| ODP-40 4D ODP-40 4E |
(1)0.75micro-L/mL n-Hexyl benzoate (2)6micro-L (3)CH3CN/H2O=65/35 (4)0.6mL/min (5)30deg-C (6)UV(254nm) |
| C8P-50 4D C8P-50 4E |
(1)0.75micro-L/mL n-Hexyl benzoate (2)6micro-L(C8P-50 4D), 10micro-L(C8P-50 4E) (3)CH3CN/H2O=65/35 (4)0.6mL/min (5) 30deg-C (6)UV(254nm) |
| C4P-50 4D C4P-50 4E |
(1)0.75micro-L/mL n-Hexyl benzoate (2)6micro-L (3)CH3CN/H2O=65/35 (4)0.6mL/min (5) 30deg-C (6)UV(254nm) |
| ODP2 HP-4B ODP2 HP-4D ODP2 HP-4E ODP2 HP-2B ODP2 HP-2D |
(1)1,000mg/L Toluene (2)5micro-L(ODP2 HP-4B, ODP2 HP-4D, ODP2 HP-4E), 2micro-L(ODP2 HP-2B), 1micro-L(ODP2 HP-2D) (3)H2O/CH3CN=55/45 (4)0.5mL/min(ODP2 HP-4B, ODP2 HP-4D, ODP2 HP-4E), 0.1mL/min(ODP2 HP-2B, ODP2 HP-2D) (5)40deg-C (6)UV(254nm) |
| RP18-415 | (1)1% Acetone (2)3micro-L (3) CH3CN/H2O=95/5 (4)1.0mL/min (5)Ambient (6)UV(254nm) |
| RP18-613 RP18-413 |
(1)1% 3-Pentanone (2)5micro-L (3) CH3CN/THF/H2O=30/30/40 (4)0.8mL/min(RP18-613), 0.6mL/min(RP18-413) (5)Ambient (6)UV(262nm) |
| DS-613 | (1)0.1% Benzene (2)20micro-L (3) CH3CN/THF/H2O=40/30/30 (4)0.8mL/min (5)Ambient (6)UV(254nm) |
| DS-413 | (1)1% 3-Pentanone (2)5micro-L (3) CH3CN/THF/H2O=30/30/40 (4)0.6mL/min (5)Ambient (6)UV(262nm) |
| DE-613 | (1)0.2% 1,5-Pentanediol (2)20micro-L (3) H2O (4)0.8mL/min (5)Ambient (6)RI |
| DE-413 DE-413L DE-413S |
(1)0.6% Di-n-butyl ketone (2)10micro-L(DE-413), 20micro-L(DE-413L), 8micro-L(DE-413S) (3) CH3CN/H2O=50/50 (4)1.0mL/min (5)40deg-C(DE-413, DE-413L), Ambient(DE-413S) (6)UV (262nm) |
| DE-213 | (1)0.6% Di-n-butyl ketone (2)5micro-L (3) CH3CN/H2O=50/50 (4)0.2mL/min (5)40deg-C (6)UV (262nm) |
| GOLF-413 CARB-413 CARB-413L |
(1)0.6% Di-n-butyl ketone (2)10micro-L (3) CH3CN/H2O=50/50 (4)1.0mL/min (5)40deg-C (6)UV (262nm) |
| DM-614 | (1)2% Oxalic acid (2)5micro-L (3)5mM H3PO4 aq. (4)0.8mL/min (5)Ambient (6)RI |
| NN-814 NN-614 NN-414 |
(1)0.1% Uracil (2)10micro-L(NN-814), 7micro-L(NN-614, NN-414) (3)0.1M NaH2PO4(pH3.0) (4)1.0mL/min(NN-814), 0.8mL/min(NN-614), 0.3mL/min(NN-414) (5)35deg-C (6)UV(260nm)(NN-814, NN-614), RI(NN-414) |
| JJ-50 4D JJ-50 2D |
(1)1.0% Acetone (2)2micro-L(JJ-4D), 0.5micro-L(JJ-2D) (3)CH3CN/H2O=60/40 (4)1.0mL/min(JJ-4D), 0.15mL/min(JJ-2D) (5)Ambient (6)UV(280nm) |
| NH2P-50 4D NH2P-50 4E NH2P-50 2D |
(1)5mg/mL Sucrose (2)6micro-L(NH2P-50 4D, NH2P-50 4E), 1.5micro-L(NH2P-50 2D) (3)CH3CN/H2O=75/25 (4)0.6mL/min(NH2P-50 4D), 1.0mL/min(NH2P-50 4E), 0.2mL/min(NH2P-50 2D) (5)30deg-C(NH2P-50 4D, NH2P-50 4E), 35deg-C(NH2P-50 2D) (6)RI |
| F-411/S F-411 F-511 |
(1)0.15% t-Butyl benzene (2)20micro-L(F-411/S), 15micro-L(F-411, F-511) (3)CH3OH/H2O=80/20 (4)1.0mL/min (5)Ambient (6)UV(254nm) |
| C18M 4D C18M 4E C18P 4D C18P 4E |
(1)0.61mg/mL Naphthalene (2)1.5micro-L(C18M 4D, C18P 4D), 2micro-L(C18M 4E, C18P 4E) (3)CH3OH/H2O=70/30 (4)1.0mL/min (5)30deg-C (6)UV(254nm) |
| C18M 2D C18M 2E |
(1)3.05mg/mL Naphthalene (2)0.2micro-L (3)CH3CN/H2O=60/40 (4)0.2mL/min (5)30deg-C (6)UV(254nm) |
| E-411 | |
| 5SIL 4D 5SIL 4E |
(1)0.5mg/mL p-Nitrobenzyl alcohol (2)1.5micro-L(5SIL 4D), 2micro-L(5SIL 4E) (3)Hexane/C2H5OH=95/5 (4)1.0mL/min (5)30deg-C (6)UV(254nm) |
| 5C8 4D 5C8 4E |
(1)0.61mg/mL Naphthalene (2)1.5micro-L(5C8 4D), 2micro-L(5C8 4E) (3)CH3OH/H2O=66/34 (4)1.0mL/min (5)30deg-C (6)UV(254nm) |
| 5C4 4D | (1)0.61mg/mL Naphthalene (2)1.5micro-L (3)CH3OH/H2O=55/45 (4)1.0mL/min (5)30deg-C (6)UV(254nm) |
| 5CN 4D 5CN 4E |
(1)0.60mg/mL Naphthalene (2)1.5micro-L(5CN 4D), 2micro-L(5CN 4E) (3)CH3OH/H2O=40/60 (4)1.0mL/min (5)30deg-C (6)UV(254nm) |
| 5NPE 4D | (1)0.61mg/mL Naphthalene (2)1.5micro-L (3)CH3OH/H2O=55/45 (4)1.0mL/min (5)30deg-C (6)UV(254nm) |
| 5NH 4D | (1)3.47mg/mL p-Nitrophenyl-α-D-glucopyranoside (2)1.5micro-L (3)CH3CN/H2O=95/5 (4)1.0mL/min (5)30deg-C (6)UV(254nm) |