Ribosomel RNA (1) (DEAE-420N)

Ribosomel RNA is analyzed using IEC DEAE-420N (a column for weak anion exchange chromatography packed with non-porous gel).
DEAE-420N is an old type of DEAE3N-4T.

Sample : 5s-RNA (E.Coli MRE600)

Column       : Shodex IEC DEAE-420N (4.6mmID*35mm)
Eluent       : (A); 25mM Tris-HCl buffer(pH7.5) 
               (B); (A) + 1M NaCl
               Linear gradient: 0min to 15min, 10% (B) to 100% (B)
Flow rate    : 1.5mL/min
Detector     : UV(260nm)
Column temp  : 60deg-C