Shodex PROTEIN KW-803 (a column for aqueous SEC (GFC)) shows good efficiency until 0.5mg of BSA (Bovine serum albumin) injection. If the injection to KW-803 exceeds 1mg, chromatogram of BSA becomes gradually broad. Recommended sample loading is less than 0.5mg of BSA per column.
Sample : BSA, 100micro-L
Column : Shodex PROTEIN KW-803 (8.0mmID*300mm)
Eluent : 50mM Phosphate buffer(pH7.0) + 150mM NaCl
Flow rate : 1.0mL/min
Detector : UV(280nm)
Column temp. : Room temp.