Standard Proteins (10) (DEAE-825, DEAE3N-4T)
Three kinds of protein standards were analyzed using IEC DEAE3N-4T (a column for weak anion exchange chromatography packed with non-porous gel). Fast analysis is possible using DEAE-420N compared with using IEC DEAE-825 (a column for weak anion exchange chromatography packed with porous gel).
Sample :
1. Conalbumin 0.4mg/mL
2. Ovalbumin 1.2mg/mL
3. Trypsin inhibitor 1.0mg/mL
Columns : Shodex IEC DEAE-825 (8.0mmID*75mm) : Shodex IEC DEAE3N-4T (4.6mmID*35mm) Eluent : (A); 20mM Piperazine-HCl buffer(pH6.0) : (A); 25mM Piperazine-HCl buffer(pH6.0) (B); (A) + 0.5M NaCl (B); (A) + 0.5M NaCl Linear gradient: 0min to 60min, (A) to (B) Linear gradient: 0min to 10min, (A) to (B) Flow rate : 1.0mL/min : 1.5mL/min Detector : UV(280nm) Column temp. : Room temp.