Standard Proteins (11) (PH-814)
Six kinds of protein standards were analyzed using HIC PH-814 ( a hydrophobic interaction column ).
Sample :
1. Cytochrome c
2. Myoglobin
3. Ribonuclease A
4. Ovalbumin
5. Lysozyme
6. alpha Chymotrypsinogen
Column : Shodex HIC PH-814 (8.0mmID*75mm) Eluent : (A); (B) + 1.8M Ammonium sulfate (B); 0.1M Phosphate buffer(pH7.0) Linear gradient: 0min to 60min, (A) to (B) Flow rate : 1.0mL/min Detector : UV(280nm) Column temp. : Room temp.