Standard Proteins (18) (KW-803)

When a sample contains high molecular weight portions, PROTEIN KW-803 (a column for aqueous SEC (GFC)) will give a better result than Company A column.

Sample : Gel Filtration Standard (Bio Rad)

Peak	Protein	PROTEIN KW-803			Company A column
Peak	Protein	Rt(min)	Width(min)	Rs	Rt(min)	Width(min)	Rs
1	Thyroglobulin	6.5657	0.7550		6.5657	0.7550
	(MW: 670,000)			2.59			2.27
2	gamma-Globulin	8.2957	0.5825		7.7203	0.8450
	(MW: 158,000)			2.33			1.84
3	Ovalbumin	9.5547	0.5000		8.9840	0.5250
	(MW: 44,000)			1.93			2.39
4	Myoglobin	10.4380	0.4175		10.1290	0.4350
	(MW: 17,000)			4.69			3.57
5	Vitamin B₁₂	12.3423	0.3950		11.5647	0.3700
	(MW: 1,350)

Columns      : (Left); Shodex PROTEIN KW-803 (8.0mmID*300mm), (Right); Company A column
Eluent       : 50mM Sodium phosphate buffer(pH7.0) + 0.3M NaCl
Flow rate    : 1.0mL/min
Detector     : UV(220nm)
Column temp. : Room temp.