Standard Proteins (3) (RP18-415)
RSpak RP18-415 (a column for reversed phase chromatography) can analyze a variety of proteins with good recovery. This column is suitable for fast analysis at high flow rates since the maximum working pressure is 22Mpa.

|
Recovery of Proteins
|
|
|---|---|
| 1. Ribonuclease A |
93%
|
| 2. Insulin |
98%
|
| 3. Cytochrome c |
100%
|
| 4. Lysozyme |
100%
|
| 5. BSA |
98%
|
| 6. Myoglobin |
108%
|
| 7. Ovalbumin |
—
|
Column : Shodex RSpak RP18-415 (4.6mmID*150mm)
Eluent : (A); 0.1% TFA aq./CH3CN=99/1
(B); 0.1% TFA aq./CH3CN=5/95
Linear gradient: 0 to 25min, 20% (B) to 60% (B)
Flow rate : 1.0mL/min
Detector : UV(220nm)
Column temp. : Room temp.
