The study of Injection Volume ; GS-320 Series Columns
The figure shows the effect of the injection volume to the separation for the analysis of uracil using GS-320 series columns.
(Recommended injection volume)
| Product name |
Size (mm) ID x L |
Injection volume (micro-L) |
|---|---|---|
| GS320A-1E |
1.0 x 250 |
1
|
| GS320A-2E |
2.0 x 250
|
10
|
| GS-320 HQ |
7.5 x 300
|
100
|
Sample : Uracil
2ng (GS320A-1E)
10ng (GS320A-2E)
200ng (GS-320 HQ)
Columns : Shodex GS320A-1E (1.0mmID*250mm)
Shodex GS320A-2E (2.0mmID*250mm)
Shodex Asahipak GS-320 HQ (7.5mmID*300mm)
Eluent : 10mM HCOONH4 buffer(pH4.0)
Detector : UV(260nm) cell volume; 3micro-L, optical path length; 7mm
Tubing : 0.1mmID PEEK tube
Injector : Rheodyne 8125