Transfer RNA (1) (DEAE-825)

IEC DEAE-825 packed with porous gel can allow larger sample loads than IEC DEAE3N-4T packed with non-porous gel.

Sample :
Transfer ribonucleic acid, tRNA (from E.Coli)

Column       : Shodex IEC DEAE-825 (8.0mmID*75mm)
Eluent       : (A); 20mM Sodium phosphate buffer(pH6.6) + 250mM KCl + 5M Urea + 0.5mM EDTA 
               (B); (A) + 250mM KCl
               Linear gradient: 0min to 150min, (A) to (B)
Flow rate    : 1.0mL/min
Detector     : UV(260nm) 
Column temp. : Room temp.