Transfer RNA (3) (SB-805 HQ)

Transfer ribonucleic acids were analyzed using OHpak SB-805 HQ (a column for GFC separation).
Refer to Transfer RNA (2) (SB-804 HQ)

Sample :
1. 23s-RNA(E.Coli MRE 600)
2. 16s-RNA(E.Coli MRE 600)
3. 5s-RNA(E.Coli MRE 600)
4. Transfer ribonucleic acid, tRNA(E.Coli MRE 600)

Columns      : Shodex OHpak SB-805 HQ  (8.0mmID*300mm) x 2
Eluent       : 0.1M CH3COONa buffer(pH7.0) + 0.3M NaCl + 1mM EDTA
Flow rate    : 0.1mL/min
Detector     : UV(260nm)
Column temp. : Room temp.