Transfer RNA (4) (DEAE-420N)

Transfer ribonucleic acids were analyzed using IEC DEAE-420N (a column for weak anion exchange chromatography packed with non-porous gel).
DEAE-420N is an old type of DEAE3N-4T.

Sample : tRNA (E.Coli MRE600)

Column       : Shodex IEC DEAE-420N (4.6mmID*35mm)
Eluent       : (A); 25mM Tris-HCl(pH7.5) (B); (A) + 1M NaCl
               Linear gradient: 20%(B) to 50%(B), 0 to 20min
Flow rate    : 0.5mL/min
Detector     : UV(260nm)
Column temp. : 60deg-C