Trypsin and Trypsinogen

Two trypsins, alpha and beta were separated using HIC PH-814 ( a hydrophobic interaction column ).

Sample :
1. alpha Trypsin
2. Trypsinogen
3. beta Trypsin

Column       : Shodex HIC PH-814 (8.0mmID*75mm)
Eluent       : (A); (B) + 1.8M Ammonium sulfate
               (B); 0.1M Phosphate buffer(pH3.0)
               Linear gradient: 0min to 60min, (A) to (B)
Flow rate    : 1.0mL/min
Detector     : UV(280nm)
Column temp. : 25deg-C