Trypsin (Type I)

Ovomucoid is a glycoprotein exists in egg white and is a trypsin inhibitor. AFpak AOV-894 which has ovomucoid as the ligand was used for the adsorption and elution of trypsin.

Sample :Trypsin (type I)

Column       : Shodex AFpak AOV-894 (8.0mmID*50mm)
Eluent       : (A); 0.1M Sodium acetate buffer(pH7.7) + 0.5M NaCl
               (B); 0.1M Sodium acetate buffer(pH2.8) + 0.5M NaCl
               Step gradient: (A) to (B)
Flow rate    : 1.0mL/min
Detector     : UV(280nm)
Column temp. : Room temp.