Tryptic Digest of Myoglobin (1) (RP18-415)

Having high resolution, RSpak RP18-415 is suitable for the analysis of complex sample mixtures. Its performance is excellent in determining amino acid sequences in digestive protease of protein.

Sample : Triptic degest of Myoglobin

Column       : Shodex RSpak RP18-415 (4.6mmID*150mm)
Eluent       : (A); 0.1% TFA aq./CH3CN=99/1
               (B); 0.1% TFA aq./CH3CN=5/95
               Linear gradient: 0 to 30min, (A) to 50% (B)
Flow rate    : 0.6mL/min
Detector     : UV(220nm)
Column temp. : Room temp.