Having high resolution, RSpak RP18-415 is suitable for the analysis of complex sample mixtures. Its performance is excellent in determining amino acid sequences in digestive protease of protein.
Sample : Triptic degest of Myoglobin
Column : Shodex RSpak RP18-415 (4.6mmID*150mm)
Eluent : (A); 0.1% TFA aq./CH3CN=99/1
(B); 0.1% TFA aq./CH3CN=5/95
Linear gradient: 0 to 30min, (A) to 50% (B)
Flow rate : 0.6mL/min
Detector : UV(220nm)
Column temp. : Room temp.