Tryptic Digest of Myoglobin (2) (ODP-50)

Tryptic digest of myoglobin were analyzed using Asahipak ODP-50 6D (a column for reversed phase chromatography).

Abbreviation of Amino Acids

Abbreviation of Amino Acid
A	Ala	M	Met
C	Cys	N	Asn
D	Asp	P	Pro
E	Glu	Q	Gln
F	Phe	R	Arg
G	Gly	S	Ser
H	His	T	Thr
I	Ile	V	Val
K	Lys	W	Trp
L	Leu	Y	Tyr

Sample : Tryptic digest of Myoglobin

Column       : Shodex Asahipak ODP-50 6D (6.0mmID*150mm)
Eluent       : (A); 0.07% TFA aq.
               (B); CH₃CN containing 0.05% TFA
               Initial ratio (A)/(B)=90/10
               Linear gradient of 1%/min
Flow rate    : 0.8mL/min
Detector     : UV(214nm)
Column temp. : 30deg-C

Coutesy of Dr. Takano, Institute for Protein Research, Osaka University