Urease was analyzed using IEC DEAE-420N (a column for weak anion exchange chromatography packed with non-porous gel).
DEAE-420N is an old type of DEAE3N-4T.

Sample : Urease

Column       : Shodex IEC DEAE-420N (4.6mmID*35mm)             
Eluent       : (A); 20mM Tris-HCl buffer(pH8.0) 
               (B); (A) + 0.5M NaCl    
               Linear gradient: 0min to 10min, (A) to (B)     
Flow rate    : 1.5mL/min                         
Detector     : UV(280nm)                  
Column temp. : Room temp.