Whey Proteins

Whey proteins were analyzed using RSpak RP18-415 (a column for reversed phase chromatography).

Sample: Whey protein
1. gamma-Lactoalbumin
2. beta-Lactoglobulin A
3. beta-Lactoglobulin B

Column       : Shodex RSpak RP18-415 (4.6mmID*150mm)
Eluent       : (A); 0.1% TFA aq.
               (B); 0.1% TFA in CH3CN
               Linear gradient: 0 to 30min, 30% (B) to 50% (B)
Flow rate    : 1.0mL/min
Detector     : UV(220nm)
Column temp. : Room temp.