BioAdvantage HL, High Load, is intended for separations requiring maximum loading and selectivity. The high carbon load, 18%, and absence of micro-pores eliminates the problems of other high capacity columns.
Column: BioAdvantage HL C18, 5µm, 4.6 x 150mm
Mobile Phase: A: 0.1% TFA in Water B: 0.1% TFA in Acetonitrile
Gradient: Linear gradient, 1 ml/min, 0 to 30% solvent B in 30 minutes.
Detection: UV 210 nm
Discussion: The Canadian corporation, SPI, produces a well characterized, challenging peptide mixture that is very useful for quantitying HPLC column performance for peptides. The difficult to separate first pair of decapeptides are near base line resolved on Advantage HL C18 columns.