Guanine and Tryptophan

Guanine and tryptophan were analyzed using three columns for reversed phase chromtography, RSpak DM-614, NN-814 and DE-613.

Sample : 1. Guanine , 2. Tryptophan

Columns      : (left);   Shodex RSpak DM-G (4.6mmID*10mm) + DM-614 (6.0mmID*150mm)
               (Center); Shodex RSpak NN-G (6.0mmID*50mm) + NN-814 (8.0mmID*250mm)
               (Right);  Shodex RSpak DE-G (4.6mmID*10mm) + DE-613 (6.0mmID*150mm)
Eluent       : (Left);   0.3M K2HPO4(pH8.6)  
               (Center); 0.1M NaH2PO4(pH3.0 adjusted by H3PO4)
               (Right);  0.23M K2HPO4 aq./CH3OH=80/20
Flow rate    : (Left); 0.8mL/min, (Center); 1.0mL/min, (Right); 0.8mL/min
Detector     : (Left); UV(278nm), (Center); Shodex RI, (Right); UV(278nm)
Column temp. : (Left); 30deg-C, (Center); 50deg-C, (Right); 30deg-C