SUGAR SH1011 is used for samples of low molecular weight and SUGAR SH1821 packed with material of larger pore size is used for samples containing oligosaccharides. The results of analysis of samples containing saccharides, organic acids and alcohols by SH1011 and SH1811 are shown here. With RI detection, the peaks of these components overlap each other. However, with UV detection (210 nm), there is a possibility of quantifying organic acids as saccharides have little absorbance at 210 nm.
Sample : Fermentation by-products model solution
1. Dp7, 2. Dp6, 3. Dp5, 4. Dp4, 5. Dp3, 6. Dp2,
7. Glucose, 8. Fructose, 9. impurity from Lactic acid, 10. Lactic acid, 11. Glycerol
12. Acetic acid, 13. Methanol, 14. Ethanol, 15. Acetone, Butyric acid, 16. Propanol
Columns : (A) Shodex SUGAR SH-G (6.0mmID*50mm) + SH1011 (8.0mmID*300mm)
(B) Shodex SUGAR SH-G (6.0mmID*50mm) + SH1821 (8.0mmID*300mm)
Eluent : 5mM H2SO4 aq.
Flow rate : 0.75mL/min
Detector : Shodex RI, UV(210nm)
Column temp. : 50deg-C