Recovery of Proteins and Enzyme Activity (CM-825)
The tables show good recovery of IEC CM-825 ( a column for weak cation exchange chromatography ) for proteins and enzyme activity.
Recovery of proteins | |
---|---|
Protein | Recovery(%) |
alpha Chymotrypsinogen A | 90 |
Myoglobin | 97 |
Ribonuclease A | 100 |
Cytochrome c | 101 |
Lysozyme | 99 |
Recovery of enzyme activity | |
---|---|
Enzyme | Recovery(%) |
alpha Chymotrypsin | 97 |
Lysozyme | 102 |
Sample load : 0.5mg each
Column : Shodex IEC CM-825 (8.0mmID*75mm) Eluent : (A); 20mM Sodium phosphate buffer(pH7.0) (B); (A) + 0.5M NaCl Linear gradient: 0min to 1min, (A) to (B)