Recovery of Proteins and Enzyme Activity (CM-825)

The tables show good recovery of IEC CM-825 ( a column for weak cation exchange chromatography ) for proteins and enzyme activity.

Recovery of proteins
Protein Recovery(%)
alpha Chymotrypsinogen A 90
Myoglobin 97
Ribonuclease A 100
Cytochrome c 101
Lysozyme 99
Recovery of enzyme activity
Enzyme Recovery(%)
alpha Chymotrypsin 97
Lysozyme 102

Sample load : 0.5mg each

Column : Shodex IEC CM-825 (8.0mmID*75mm)

Eluent : (A); 20mM Sodium phosphate buffer(pH7.0)

         (B); (A) + 0.5M NaCl

         Linear gradient: 0min to 1min, (A) to (B)