Ribonuclease A from Equine Pancreas

Ribonuclease A from equine pancreas was analyzed using IEC CM-825 ( a column for weak cation exchange chromatography ). This enzyme contains rather small impurities.

Sample: 0.5% Ribonuclease A (pI=9.3), 30micro-L

Column       : Shodex IEC CM-825 (8.0mmID*75mm)
Eluent       : (A); 20mM Sodium phosphate buffer(pH7.0)
               (B); (A) + 0.5M NaCl
               Linear gradient: 0min to 60min, (A) to (B)
Flow rate    : 1.0mL/min
Detector     : UV(280nm)
Column temp. : 25deg-C